Part:BBa_K2717000

gdh

Sequence of glucose dehydrogenase.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1542
  
• 1000
  COMPATIBLE WITH RFC[1000]

BNUZ-China 2021

To explore whether GDH can effectively promote the proliferation of engineered bacteria without IPTG induction, we have drawn the growth curve of engineered bacteria and normal bacteria. From the growth curve (Figure 1) we can learn that compared with E. coli that had not overexpressed GDH, our engineered bacteria had a certain proliferation advantage, although the effect is not obvious. We subsequently established a mathematical model of intestinal colony competition. The results showed that although GDH slightly enhanced the colonization advantage of engineered bacteria, it could be eventually well colonized in the intestinal tract.

Figure 1. GDH overexpression promotes cell growth